Journal: Frontiers in Plant Science

Article Title: The Extracellular Superoxide Dismutase Sod5 From Fusarium oxysporum Is Localized in Response to External Stimuli and Contributes to Fungal Pathogenicity

doi: 10.3389/fpls.2021.608861

Figure Lengend Snippet: The SP and GPI anchor are required for FoSod5 subcellular localization within Fov . (A) Western blot analysis with anti-GFP of total protein found in the culture medium of FoSod5 derivatives and the WT. Coomassie protein staining indicated the same amount of loaded protein samples. (B) Phialide localization of FoSod5-SP-GFP and FoSod5-SP-GFP-GPI by fluorescence microscopy. (C) Statistical analysis of phialide localization among FoSod5-SP-GFP and FoSod5-SP-GFP-GPI, where n represents the number of phialides counted at a given time. Three counts from independent biological samples were used for the statistical analysis. Scale bars represents 10 μm.

Article Snippet: Anti-GFP antibody (Rockland Immunochemicals, Gilbertsville, PA, United States) and an ECL chemiluminescent detection kit (GE Healthcare) were used to detect the resulting proteins.

Techniques: Western Blot, Staining, Fluorescence, Microscopy